Figure 1.

A GCN4/gcn4Δ strain is haploinsufficient for growth in the presence of SMM, ETH, and CAN. Ten-fold serial dilutions of wild-type BY4743 or GCN4/gcn4Δ cells were plated onto complete or amino acid starvation medium. (A) Cells were plated onto SC control plates and SC-ile-val plates containing SMM at the concentrations shown to induce starvation for isoleucine and valine. (B) Cells were plated onto SC-met-cys control plates (see the results section Identification of genes causing haploinsufficiency for growth on SMM for details) and SC-met-cys plates containing ETH at the concentrations shown to induce starvation for methionine. (C) Cells were plated onto SC control plates and SC-arg plates containing CAN at the concentrations shown to induce starvation for arginine.