Figure 5.

Examples of ETH and CAN haploinsufficient phenotypes. (A) ETH phenotypes of all phenotypically Met⁺ strains were tested. The cells were treated as in Figure 4B, except that they were plated onto SC-met-cys + ETH (10 and 15 µg/ml are shown). The MON2/mon2Δ strain grows similarly to the BY4743 wild type. The RPL33A/rpl33aΔ, YPL142C/ypl142cΔ, and TAF14/taf14Δ strains are sensitive to ETH. (B) All SMM-sensitive strains were tested for growth in the presence of CAN. Cells were treated as in Figure 3A, except they were plated onto SC-arg + CAN (3 and 4 µg/ml are shown). The met15Δ::kanMX4/met15Δ0 strain is no more sensitive to CAN than the BY4743 wild type (top panels). The RPL33A/rpl33aΔ and YPL142C/ypl142cΔ strains are both CAN-sensitive but the TAF14/taf14Δ strain is not.