Figure 4.

Plots of recombination frequency (RF_M) estimated from phased genotype data by starting at the terminal markers at the beginning (red points) and end (blue points) of each linkage and recording the proportion of offspring with an observed recombination (i.e., change of phase) in the interval between the terminal marker (m₀) and each subsequent marker (m_n) for the 12 cranberry LGs. Centromere spans (gray regions) were placed on the 12 LGs of the cranberry parental component bin maps constructed for the parents of the CNJ04 population, Mullica Queen (a) and Stevens (b); the CNJ02 population, Mullica Queen (c) and Crimson Queen (d); and the GRYG population, [BGx(BLxNL)]95 (e) and GH1x35 (f) using the method developed in Limborg et al. (2016). Centromeric spans in the LGs were defined as the range (centimorgans) extending from the intersection (dark lines) of the recombination frequency (RF_M) estimates made from both ends of the LG outwards until reaching the first marker with an RF_M = 0.45 in both directions. (g) Marker density in the cranberry composite map is shown to explore the relationship between marker density and centromere position.