Figure 1.

Identification of ESTs corresponding to the wheat homeologs. (A) Contig assembly of wheat homeolog ESTs corresponding to a physically mapped EST (marked red) used as a query (Q). Green bars represent the wheat ESTs identified using a query EST and blue bars denote the additional wheat ESTs identified using the corresponding rice full-length (orange bar) cDNA sequence as a bridge. (B) Sequence alignment of wheat ESTs showing different sequence patterns, each corresponding to a homeolog. ESTs showing a similar pattern are bracketed and marked by different color brackets (green, red, and blue). Black arrows mark the conserved region used to design PCR primers for the SSCP analysis. cDNA, complementary DNA; EST, expressed sequence tag; PCR, polymerase chain reaction; SSCP, single-stranded conformational polymorphism.