Fig. 2. Immunohistochemical staining of phosphorylated glycogen synthase kinase-3β (p-GSK-3β) in the spinal cords of normal (A) and experimental autoimmune encephalomyelitis (EAE) rats on day 13 postimmunization (PI) (G.3) (B–D) and day 21 PI (R.0) (E). p-GSK-3β in astrocytes (arrows) in the spinal cords of normal controls. On day 13 PI, p-GSK-3β was expressed by infiltrating inflammatory cells (B and E, arrowheads), astrocytes (B and E, arrows), neurons (C, arrows), vascular endothelial cells (D, “V”) and ependymal cells (D) in the spinal cord. Primary antibody was omitted (F). Counterstained with hematoxylin. Scale bars=25 µm (A–F).