FIG. 2.

siRNA-mediated inhibition of LACV replication. The percent decrease in LACV titer in LACV siRNA-treated cells was compared to that in mock-transfected cells at 48 h for infections with various MOIs (0.01, 0.001, and 0.0001 PFU/cell). The pretreatment of 293T cells with LACV siRNAs resulted in an up to 99% inhibition of virus replication when the cells were challenged with LACV at a low MOI (0.0001). The efficiency of LACV siRNAs decreased as cells were challenged with increasing MOIs (0.001 and 0.01). Error bars represent variabilities (SEM) between triplicate samples for each time point.