FIG. 4.

LACV glycoprotein expression is decreased in 293T cells that are pretreated with LACV siRNAs. LACV antigen accumulation in 293T (A to C) and C6/36 (D to F) cells was measured by FACS with a panel of LACV G1-specific antibodies 48 h after siRNA transfection. As shown in these representative plots, LACV-specific siRNAs, including M1627 (A and D) and S356 (B and E), inhibited LACV G1 expression. The LACV control siRNA 528c (C and F) did not affect LACV glycoprotein expression in 293T cells. LACV siRNAs did not decrease TAHV glycoprotein expression in either 293T or C6/36 cells, with the exception of M2860 (data not shown).