FIG. 5.

Bulk-phase endocytosis is unaffected in PVM-infected immature DCs. Alexa Fluor 568-conjugated dextran was incubated with either mock-infected (A) or infected (B to G) cells at 4 or 37°C for 1 h at various times p.i. Cells were also stained with rabbit anti-PV capsid antibodies and examined with a confocal microscope. (A) Mock-infected DCs and dextran at 37°C. (B) Dextran uptake (left panel) and PVM staining (right panel) of infected DCs at 4°C at 0 h p.i. (C to G) Dextran uptake (upper panels) and PVM staining (lower panels) of infected DCs at 2 (C), 3 (D), 4 (E), 5 (F), or 6 (G) h p.i. Magnification, ×100.