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. 2017 Mar 6;174(9):809–821. doi: 10.1111/bph.13739

Figure 5.

Figure 5

Expression of components of the SDF‐1‐CXCR4/CXCR7 axes in immortalized human podocytes. (A) Expression of the gene encoding for SDF‐1, CXCR4 and CXCR7 was evaluated in immortalized human podocytes by RT‐PCR analyses. Actin (ACTIN) was adopted as an internal standard to control for unwanted sources of variation. Each image is representative of five experiments run in duplicate for each experimental group. (B) Release of SDF‐1α by immortalized human podocytes was evaluated by measuring concentrations of this chemokine in fresh (0 days; baseline) and podocyte‐conditioned (5 days) media by ELISA. Data are expressed as mean ± SEM of five experiments run in triplicate for each experimental group. *P < 0.05 versus baseline.