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Effects of linagliptin and AMD3100 on ERK1/2 activation in immortalized human podocytes. Immortalized human podocytes were exposed to vehicle alone (control), linagliptin (1 nM), AMD3100 (0.3 μM) or both for 18 h. ERK1/2 activation was evaluated by western blot analyses. β‐Actin was adopted as an internal standard to control for unwanted sources of variation. Each image is representative of five experiments run in duplicate for each experimental group.
