Figure 8.

Effects of AZD6244 on the growth of immortalized human podocytes. Immortalized human podocytes were exposed to vehicle alone (control), increasing AZD6244 concentrations (0.1–10 μM; 5 days), in the absence or presence of linagliptin (1–100 nM), and cell growth was measured by a colorimetric assay. To set the Y axis, all values were normalized to the mean value of the control group (0%). Data are the mean ± SEM of five experiments run in triplicate for each experimental group. Solid lines represent the combined observed effects. Dashed lines represent the combined effects predicted by assuming Bliss independence.