Figure 2.

Comparison of the effects of eucalyptol on CFA‐induced paw oedema in wild‐type and Trpm8^−/− mice, with animals treated as in Figure 1A. (A) Representative photographs of CFA‐induced paw oedema in wild‐type (top row) or Trpm8^−/− mice (bottom row) injected i.p. with vehicle (Veh, corn oil) or 300 mg·kg⁻¹ eucalyptol (Euc), taken 50 h after intraplantar CFA injection. (B) Time course of CFA‐induced paw oedema in wild‐type mice, treated with vehicle, eucalyptol (300 mg·kg⁻¹) or the reference drug ibuprofen (Ibu, 30 mg·kg⁻¹). Black arrows indicate the time points of treatment. (C) Time course of CFA‐induced paw oedema in Trpm8^−/− mice. (D) Comparisons of weights of paw biopsies from wild‐type and Trpm8^−/− mice of control group (no CFA), CFA groups treated with vehicle, eucalyptol or ibuprofen respectively. Tissues were collected 50 h after CFA injection from mice tested in Figure 2B, C. n = 6–8 mice per group. ^# P < 0.05, significantly different from control group, *P < 0.05, significantly different from CFA + Veh group, NS, no significance (P > 0.05), one‐way ANOVA.