Figure 4.
Influence of eucalyptol treatment on CFA‐induced inflammatory cytokines in hind paw skin of wild‐type and Trpm8−/− mice. Mice were treated (i.p.) with vehicle (Veh, corn oil), eucalyptol (Euc, 300 mg·kg−1) or ibuprofen (Ibu, 30 mg·kg−1) as in Figure 1A. Hind paw biopsies were collected 50 h after CFA injection, and levels of IL‐1β, IL‐6 and TNF‐α were determined by ELISA in tissue homogenates. # P < 0.05, significantly different from control group; *P < 0.05, significantly different from CFA + Veh group, NS, no significance (P > 0.05), one‐way ANOVA. n = 6–12 mice per group.