Table 4.
Phenotypic properties that differentiate Streptomyces isolate H9T from the type strains of Streptomyces melanogenes, Streptomyces noboritoensis and Streptomyces polyantibioticus
| Phenotypic tests | Isolate H9T |
S. melanogenes
NRRL B- 2072T |
S. noboritoensis NRRL B-12152T |
S. polyantibioticus
NRRL B-24448T |
|---|---|---|---|---|
| Morphology | ||||
| Spore chains | Spiral | Straight to flexuousa | Straight to flexuousb | Spores held within sporangiac |
| API ZYM tests | ||||
| α-Chymotrypsin | – | – | – | + |
| Esterase (C4) | + | – | + | + |
| β-Galactosidase | + | + | – | + |
| α-Glucuronidase | + | + | – | + |
| GEN III BIOLOG microplates | ||||
| Oxidation of: | ||||
| N-acetyl-d-Galactosamine, d-fructose, inosine, d-mannose |
+ | + | + | – |
| l-Arginine | – | – | + | + |
| d-Aspartic acid, N-acetyl-β- d-mannosamine |
– | + | – | + |
| Citric acid | – | + | + | + |
| d-Fructose-6-phosphate | – | + | – | + |
| d-Fucose, d-raffinose | + | – | – | – |
| Guanidine | – | – | + | – |
| β-hydroxy-Butyric acid | + | – | + | + |
| β-methyl-d-Glucoside | – | + | – | – |
| Pectin | + | – | – | – |
| l-Pyroglutamic acid | + | – | – | + |
| d-Salicin | – | – | – | + |
| d-Turanose | + | + | – | – |
| Inhibition tests | ||||
| Sodium bromate | + | + | + | – |
| Sodium lactate (1%) | – | – | – | + |
| Tetrazolium blue | – | – | + | – |
| Growth in the presence of | ||||
| Sodium chloride (4%, w/v) | + | + | + | – |
| Growth at | ||||
| pH 5 | – | – | – | + |
| Other phenotypic tests | ||||
| Biochemical tests | ||||
| Allantoin hydrolysis | – | – | – | + |
| Urea hydrolysis | – | + | + | – |
| Degradation tests | ||||
| Arbutin | – | + | + | + |
| Casein | + | – | – | + |
| Elastin | – | + | + | + |
| Guanine | – | + | + | – |
| Hypoxanthine, l-tyrosine | – | + | + | + |
| Starch | + | – | – | + |
| Uric acid | + | + | + | – |
| Tween 80 | + | – | – | + |
| Growth at | ||||
| 40 °C | + | – | – | – |
+, positive result; −, negative result
a , b and c, data taken from Isono et al. (1957) and (le Roes-Hill and Meyers 2009), respectively
Positive results recorded for Streptomyces isolate H9T, S. melanogenes NRRL B- 2072T, S. noboritoensis NRRL B-12152T and S. polyantibioticus NRRL B-24448T
API ZYM tests: acid phosphatase, alkaline phosphatase, cystine arylamidase, esterase lipase (C8), β-glucosidase, leucine arylamidase, lipase (C14), α-mannosidase, naphthol-AS-BI-phosphohydrolase, N-acetyl-β-glucosaminidase and valine arylamidase
GEN III BIOLOG microplates: utilisation of l-alanine, l-aspartic acid, l-glutamic acid, l-histidine, l-serine (amino acids), γ-amino-n-butyric acid, α-keto-butyric acid, α-keto-glutaric acid, acetic acid acetoacetic acid, d-gluconic acid, l-malic acid, propionic acid (organic acids); glycyl-proline (peptide); d-cellobiose, dextrin, l-fucose, d-galactose, 3-O-methyl-d-galactose, β-gentiobiose, d-glucose, glycerol, d-melibiose, stachyose (sugars); d-trehalose (sugar alcohol) and growth in the presence of potassium tellurite, rifamycin SV and sodium chloride (1%, w/v)
Other phenotypic tests: aesculin hydrolysis, degradation of adenine and Tween 40 and growth at 10, 20 and 30 °C
Negative results recorded for Streptomyces isolate H9T, S. melanogenes NRRL B- 2072T, S. noboritoensis NRRL B-12152T and S. polyantibioticus NRRL B-24448T
API ZYM tests: α-fucosidase, α-galactosidase, β-glucuronidase and trypsin
GEN III BIOLOG microplates: utilisation of d-serine#1, d-serine#2, (amino acids); butyric acid, d-malic acid, mucic acid, N-acetyl-neuraminic acid, quinic acid, d-saccharic acid, (organic acids); α-d-lactose, l-rhamnose, stachyose (sugars); d-galacturonic acid, l-galactonic acid-γ-lactone, (sugar acids); d-arabitol, d-mannitol, d-salicin, d-sorbitol (sugar alcohols); glucuronamide (amino hexose) and resistance to fusidic acid, guanidine hydrochloride, lincomycin, minocycline, niaproof, sodium formate, tetrazolium violet, tetrazolium blue, troleandomycin, vancomycin and growth in the presence of sodium chloride (8%, w/v) and at pH 6
Other phenotypic tests: H2S production, nitrate reduction, degradation of cellulose, chitin, xanthine, xylan and tributyrin and growth at 4 or 45 °C