Table 1.
List of the differentially expressed proteins in spleen identified by MALDI/TOF/TOF and MS/MS analysis
| Spot ID | Protein name | Accession no. | Fold change in expression | pI, MW (kDa) | Protein score | Function | ||
|---|---|---|---|---|---|---|---|---|
| 7 dpi | 14 dpi | 21 dpi | ||||||
| Cellular structural protein | ||||||||
| 42 | Beta-actin | gi|63018 | +2.0 | +1.6 | +2.3 | 5.29/42.07 | 505 | Unknown |
| 63 | Alpha-actinin-4 (ACTN4) | gi|45384104 | -1.4 | -1.1 | N | 5.13/104.71 | 528 | Actin binding calcium ion binding |
| 7 | Actin, cytoplasmic type 5 | gi|56119084 | -2.1 | N | N | 5.30/42.15 | 265 | ATP binding |
| 17 | Actin, cytoplasmic type 5 (ACT5) | gi|56119084 | +3.3 | +1.5 | +2.6 | 5.30/42.15 | 235 | ATP binding |
| 18 | Actin, cytoplasmic type 5 (ACT5) | gi|56119084 | +2.4 | +1.4 | +3 | 5.30/42.15 | 694 | ATP binding |
| 20 | Actin, cytoplasmic type 5 (ACT5) | gi|56119084 | +2.8 | +1.5 | +2.5 | 5.30/42.15 | 735 | ATP binding |
| 21 | Actin, cytoplasmic type 5 (ACT5) | gi|56119084 | +1.7 | +1.4 | +1.9 | 5.30/42.15 | 576 | ATP binding |
| 22 | Actin, cytoplasmic type 5 (ACT5) | gi|56119084 | +6.1 | +1.9 | +3.1 | 5.30/42.15 | 868 | ATP binding |
| 23 | Actin, cytoplasmic type 5 (ACT5) | gi|56119084 | +14.9 | +5.6 | +2.3 | 5.30/42.15 | 898 | ATP binding |
| 24 | Actin, cytoplasmic type 5 (ACT5) | gi|56119084 | +5.0 | +1.8 | +2.1 | 5.30/42.15 | 715 | ATP binding |
| 25 | Actin, cytoplasmic type 5 (ACT5) | gi|56119084 | +3.0 | +1.2 | +2.4 | 5.30/42.15 | 888 | ATP binding |
| 26 | Actin, cytoplasmic type 5 (ACT5) | gi|56119084 | +5.2 | +1.2 | +2.6 | 5.30/42.15 | 808 | ATP binding |
| 27 | Actin, cytoplasmic type 5 (ACT5) | gi|56119084 | +2.2 | +1.6 | +1.8 | 5.30/42.15 | 938 | ATP binding |
| 28 | Actin, cytoplasmic type 5 (ACT5) | gi|56119084 | +3.1 | +11.5 | +2.1 | 5.30/42.15 | 781 | ATP binding |
| 29 | Actin, cytoplasmic type 5 (ACT5) | gi|56119084 | +3.6 | +1.6 | +1.8 | 5.30/42.15 | 728 | ATP binding |
| 30 | Actin, cytoplasmic type 5 (ACT5) | gi|56119084 | +9.8 | N | N | 5.30/42.15 | 100 | ATP binding |
| 31 | Actin, cytoplasmic type 5 (ACT5) | gi|56119084 | +2.5 | +2.1 | +2.3 | 5.30/42.15 | 587 | ATP binding |
| 32 | Actin, cytoplasmic type 5 (ACT5) | gi|56119084 | +3.7 | +2.2 | +2.5 | 5.30/42.15 | 81 | ATP binding |
| 33 | Actin, cytoplasmic type 5 (ACT5) | gi|56119084 | +2.0 | +1.2 | +2.1 | 5.30/42.15 | 611 | ATP binding |
| 34 | Actin, cytoplasmic type 5 (ACT5) | gi|56119084 | +2.0 | N | N | 5.30/42.15 | 650 | ATP binding |
| 13 | Actin, cytoplasmic type 5 | gi|56119084 | +3.2 | N | N | 5.30/42.15 | 235 | ATP binding |
| 36 | Actin, cytoplasmic type 5 (ACT5) | gi|56119084 | +2.5 | +2.5 | +1.9 | 5.30/42.15 | 741 | ATP binding |
| 45 | Actin, cytoplasmic type 5 (ACT5) | gi|56119084 | +8.9 | +9.4 | +4.0 | 5.30/42.15 | 837 | ATP binding |
| 53 | Actin, cytoplasmic type 5 (ACT5) | gi|56119084 | +1.6 | +1.5 | +1.3 | 5.30/42.15 | 618 | ATP binding |
| 58 | Actin, cytoplasmic type 5 (ACT5) | gi|56119084 | +2.2 | +2.5 | N | 5.30/42.15 | 523 | ATP binding |
| 9 | Vimentin | gi|212868 | -1.5 | -1.8 | -6.4 | 5.09/53.16 | 177 | Structural molecule activity |
| 12 | Vimentin | gi|212868 | +5.7 | N | N | 5.09/53.16 | 795 | Structural molecule activity |
| 55 | Lamin-B2(LMNB2) | gi|45384202 | +3.3 | N | N | 5.31/68.01 | 675 | Structural molecule activity |
| Signal transduction | ||||||||
| 41 | PREDICTED: similar to D4-GDP-dissociation inhibitor (Rho-GDI) | gi|50728568 | +1.6 | N | N | 5.08/22.92 | 595 | Rho GDP-dissociation inhibitor activity |
| 67 | Rho GDP-dissociation inhibitor 1 (Rho-GDI) | gi|53126513 | -1.6 | -1.1 | N | 5.22/23.31 | 124 | Rho GDP-dissociation inhibitor activity |
| Stress-response and immune-response-associated proteins | ||||||||
| 3 | Heat shock protein Hsp70 | gi|37590081 | -1.9 | -1.4 | -2.3 | 5.66/70.09 | 949 | Stress response |
| 6 | Heat shock cognate 71 kDa protein (HSPA8) | gi|45384370 | +3.0 | N | N | 5.47/71.01 | 591 | Stress response |
| 16 | 60 kDa heat shock protein, mitochondrial precursor (Hsp60) | gi|61098372 | +2.0 | +2.0 | N | 5.72/61.10 | 695 | Chaperone |
| 19* | Heat shock cognate 71 kDa protein | gi|45384370 | +4.3 | +2.1 | +2 | 5.47/71.01 | 38 | Stress response |
| 43 | Stress-70 protein, mitochondrial precursor | gi|57524986 | +1.4 | N | +2.2 | 6.09/73.43 | 68 | Cell proliferation and cellular aging |
| 60 | Heat shock protein HSP 90-alpha (Hsp90AA1) | gi|157954047 | -3.3 | -1.9 | N | 5.01/84.40 | 370 | ATP binding response to stress |
| 61 | Heat shock protein HSP 90-alpha (Hsp90AA1) | gi|157954047 | -1.4 | -1.8 | N | 5.01/84.40 | 544 | ATP binding response to stress |
| 10 | Cognin/prolyl-4-hydroxylase/protein disulfide isomerase(PDIA3) | gi|21703694 | +2.9 | +1.2 | +1.6 | 4.75/58.89 | 477 | Catalytic activity |
| 40 | Protein disulfide-isomerase A3 precursor (PDIA3) | gi|45383890 | +1.8 | N | N | 5.76/56.54 | 515 | Protein disulfide oxidoreductase activity |
| Calcium ion binding | ||||||||
| 2* | Parvalbumin (pvalb1) | gi|225877920 | +2.7 | N | N | 4.94/12.06 | 44 | Calcium ion binding |
| 50 | Annexin A6 (ANXA6) | gi|50982399 | -2.1 | -1.4 | -2.1 | 5.57/75.57 | 180 | Calcium ion binding |
| 56 | Chain A, Crystal Structures Of Chicken Annexin V | gi|62738641 | -1.6 | -1.4 | -1.7 | 5.61/36.15 | 680 | Calcium ion binding |
| Apoptosis or tumor-associated proteins | ||||||||
| 15 | Serpin B6 (SERPINB6) | gi|57530448 | +2.3 | N | N | 5.28/43.24 | 397 | Serine-type endopeptidase inhibitor activity |
| 70 | Cathepsin B (CTSB) | gi|603203 | +2.0 | +1.4 | +1.3 | 5.74/38.47 | 144 | Regulation of catalytic activity |
| 62 | Valosin containing protein | gi|90990971 | -4.0 | N | -1.48 | 5.14/89.95 | 636 | Nucleoside-triphosphatase activity |
| 37 | Ferritin H subunit | gi|21177 | +2.3 | +6.6 | +1.3 | 5.78/21.24 | 493 | Ferroxidase activity |
| Other | ||||||||
| 1 | Gamma-glutamyltransferase (TGL2) | gi|62903517 | -2.7 | N | N | 4.90/79.11 | 875 | Gamma-glutamyltransferase activity |
| 5 | Argininosuccinate synthase(ASS1) | gi|61657937 | +2.1 | +1.4 | N | 6.10/47.33 | 872 | Arginine succinate synthetase activity |
| 8 | Pre-mRNA-processing factor 19 (PRPF19) | gi|86129600 | -1.9 | N | N | 6.19/55.55 | 394 |
DNA binding DNA repair |
| 14 | PREDICTED: ribosomal protein SA isoform 1(RPSA) | gi|118086026 | +3.8 | N | N | 4.8/33.11 | 211 | Laminin receptor Activity(Rattus norvegicus) |
| 48* | Otokeratin | gi|3746660 | +1.7 | N | N | 5.97/53.77 | 56 | Structural molecule activity |
| 49 | Serum albumin precursor (VTDB) | gi|45383974 | -1.3 | N | -2.8 | 5.51/71.86 | 341 | Vitamin transporter activity |
| 57 | Chain B, Solution Nmr Structure Of V-1 Bound To Capping Protein | gi|297787504 | -1.7 | N | -1.4 | 5.5/30.71 | 457 | Actin binding |
| 59 | Alpha-tropomyosin (smooth muscle) | gi|212807 | +1.6 | N | +4.9 | 4.68/32.89 | 292 | Actin binding |
| 65 | Similar to MGC84496 protein | gi|118101652 | -1.5 | -1.4 | -2.3 | 6.07/22.80 | 176 | Unknown |
| Hypothetical proteins | ||||||||
| 4 | PREDICTED: hypothetical protein, partial | gi|118105147 | +1.1 | +1.7 | +1.4 | 4.77/66.11 | 147 | Unknown |
| 11 | Hypothetical protein | gi|53136508 | +2.0 | +1.2 | +1.9 | 6.22/53.74 | 257 | Unknown |
| 38 | Hypothetical protein | gi|53126859 | +13.8 | +7.2 | +6.0 | 5.31/42.10 | 535 | Unknown |
| 35 | Hypothetical protein | gi|53126859 | +4.1 | +3 | +2.9 | 5.31/42.10 | 650 | ATP binding |
| 51 | Hypothetical protein | gi|53126859 | -1.2 | -1.4 | -1.2 | 5.31/42.10 | 852 | Uknown |
| 52 | Hypothetical protein | gi|53126859 | +2.6 | +1.3 | +1.7 | 5.31/42.10 | 881 | Unknown |
| 54 | Hypothetical protein | gi|53126859 | -2.0 | -2.5 | -1.6 | 5.31/42.10 | 676 | Unknown |
| 69 | Hypothetical protein | gi|60098585 | -1.8 | -1.2 | -1.6 | 5.53/60.19 | 850 | Chaperone ATP binding |
a) Spot ID is the unique number that refers to the labels in Figure 3
b) Accession number: gi number in NCBI
c) Score: protein score based only on MS spectra by MALDI-TOF. Other spots were based on combined MS and MS/MS spectra from MALDI-TOF-TOF identification. A protein score greater than 83 is significant in this study (P < 0.05)
“-”, downregulated protein; “+”upregulated protein; “N”, no difference; “*”, failed identification