Fig. 1.

Generation and growth analysis of Synechocystis Δraf1 mutant. a Scheme for insertional inactivation of sll0102 gene. The 657 bp-long fragment of this gene was replaced with a kanamycin-resistance cassette. A construct was used for transformation of the wild-type cells to generate an raf1 knock-out mutant. Hybridization positions of primers used for confirmation of the introduced mutation were indicated. b Confirmation of mutation introduction (for 3 independent mutant lines) and homogeneity by PCR. c Comparison of growth rates of wild-type and Δraf1 Synechocystis cells in standard cultivation conditions monitored by measurement of OD₇₃₀ (mean OD₇₃₀ values for three independent cultures of each strain with standard deviation bars plotted against timescale)