Fig. 3.

Comparison of RbcL and RAF1 amounts and composition of RbcL- and RAF1-containing complexes in wild-type and Δraf1 Synechocystis cells subjected to sulfur starvation as well as oxidative and salt stress. A total of 20 µg of protein extracts from wild-type and mutant cells were loaded per lane, separated under denaturing (SDS-PAGE; extracts from three independent cultures were used) or non-denaturing conditions (Native-PAGE) and subjected to immunodetection using anti-RAF1 (left panel a sulfur starvation, c oxidative stress, e salt stress) or anti-RbcL (right panel b sulfur starvation, d oxidative stress, f salt stress) antibodies. As a control, protein extract from untreated wild-type cells was used