Fig. 5.

Identification of putative RAF1-interacting proteins in cyanobacterial cells. Crude protein extracts from Synechocystis sp. PCC 6803 were incubated with N- (N-ST) or C-terminally (C-ST) labeled RAF1 and subjected to a pull-down experiment using Strep-Tactin Superflow Plus resin. As a control, cell extracts without the addition of labeled RAF1 were used (C). Eluted proteins were separated under denaturing conditions and silver-stained. Separate protein bands were excised from gel and their polypeptide composition was identified by mass spectrometry. Polypeptides which are not RAF1 proteolysis or aggregation products are indicated with subsequent numbers and specified in the table