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Hemolytic activity of TB, TB_L1FK, and TB_KKG6A on human erythrocytes after 1 h of incubation at 37°C. The hemolytic activity was evaluated by the spectrophotometric determination of hemoglobin released from erythrocytes. PBS (0% hemolysis) and Triton X-100 (100% hemolysis) were used as controls. Hemolysis values ≤ 10% (dashed line) are considered to be non-hemolytic (Amin and Dannenfelser, 2006). Data are reported as mean ± standard error of three independent experiments.
