FIGURE 4.

Effect of honokiol on cell cycle progression KRAS mutant cell lines. (A) Cell cycle distribution analysis by flow cytometry. H460, A549, and H358 cells were incubated with indicated 60 μM honokiol for 24 h. The cells were collected, stained with PI and analyzed by flow cytometer. Bar diagram showing the distribution of cells in different phases of the cell cycle. (B) Western blot analysis of cycle-associated proteins. KRAS mutant cells (H460, A549, and H358) were treated with 60 μM honokiol 24 h. p21, p27, and cyclin D1 protein expression was measured by western blot. ^∗∗P < 0.01 and ^∗∗∗P < 0.001 for comparison between control group and honokiol-treated group.