Figure 5.

Synergistic effect of PDGFaa and EGF in promoting the self-renewal of O4⁻ OPCs. (A) Representative images of O4⁺ and/or BrdU⁺ cells cultured in PDGFaa or PDGFaa + EGF for 5 days, cell proliferation was analyzed by BrdU incorporation for 24 h before fixation. (B) Quantification of O4⁺ cells in PDGFaa and PDGFaa + EGF cultures. (C) Quantification of BrdU⁺ cells in PDGFaa and PDGFaa + EGF cultures. Statistical analyses are presented as mean ± SD, n = 3. *P < 0.05, **P < 0.01, Scale bars: 100 μm.