Figure 8.

EMSA results for specific DNA and metal binding by Zur. (A) 0.1 μM of 149 bp zur/znuA (lanes 1–5) or 128 bp aztD (lanes 6–10) promoter DNA was combined with 10 μM Zur, 25 μM Zn, and 100 μM EDTA in the presence or absence of a competitor oligomer of 59 bp at 5 μM containing the specific znuA/zur sequence (S), a randomized sequence (R) or the norC sequence (N). (B) 10 μM apo-Zur was used as is or incubated with 25 μM of the indicated metal prior to addition of 100 μM EDTA and 0.1 μM znuA/zur promoter DNA.