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. 2017 Apr 11;8:612. doi: 10.3389/fmicb.2017.00612

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Copyright © 2017 Widana Gamage and Dietzgen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Immunofluorescent detection of transiently expressed CaCV NSs protein in nuclei of RFP-H2B N. benthamiana protoplasts. Transgenic RFP-H2B nuclear marker protoplast preparations expressing NSs or empty vector were incubated with mouse monoclonal antibody (MAb), raised against NSs or non-specific mouse IgG1 antibody and detected using goat anti-mouse IgG conjugated with Alexa Fluor 488. Fluorescence of Alexa Fluor 488 was viewed using the GFP channel and RFP using the red channel. Bar, 10 μm.