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. 2017 Apr 11;8:612. doi: 10.3389/fmicb.2017.00612

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Copyright © 2017 Widana Gamage and Dietzgen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Intracellular localization of CaCV NSm protein and PD. RFP fusions of functional (NSm) and dysfunctional (ΔNSm) protein-expressing N. benthamiana leaves were stained with aniline blue fluorochrome to visualize PD. Enlarged sections of images are highlighted with dotted boxes. Co-localization of NSm aggregates and aniline blue are indicated with arrows. To increase contrast, aniline blue fluorescent images were false colored as cyan. Images were taken at 2 days post infiltration (dpi) using a confocal microscope at 10 × 25 magnification. Bars, 10 μm.