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Subcellular localization of HCV antigens determined by immunofluorescence. Replicon RNA-untransfected [Replicon(−)] or cloned HeLa, 293, and Huh7 [Replicon(+)] cells were cultured on coverslips, fixed in acetone-methanol, and incubated with patient serum, as described in the Materials and Methods. Representative clones of HeLa, 293, and Huh7 cells are indicated.
