Figure 1.

Construction of the slo^UAS-6b-L allele. Homologous recombination was used to construct the slo^UAS-6b-L allele. (A) Schematic representation of the inserted recombination construct is depicted above the slo transcriptional control region. (A) Recombination to the left of the 6b element inserts the Upstream Activating Sequence (UAS) sequence immediately 5′ of the 6b element and inserts a floxed mini-white gene 3′ of the 6b element. Cre recombination was used to excise the mini-white gene to produce the slo^UAS-6b-L allele. (B) Construction of the slo^6b-L control line. Recombination 3′ of element 6b inserts only the floxed mini-white gene. Cre recombination removes the mini-white gene to produce the slo^6b-L control allele of the slo gene.