Figure 5.

Mutations in the nejire gene reduce the capacity for ethanol tolerance. Recovery curves from ethanol sedation, and the respective average recovery times, for nej³ heterozygous flies (A) and nej^Q7 heterozygous flies (B), the WT Canton-S (C) and the w-/FM7 background control flies (D). In each recovery curve graph, the black curve represents recovery from a first ethanol treatment (1st exposure), whereas the gray curve represents recovery from a second ethanol treatment (2nd exposure). For each fly strain, the average recovery time of animals after the first ethanol treatment (1st exp.) or after a second ethanol treatment (2nd exp.) are shown to the right of each panel. Error bars represent SEM (Student’s t-test: *denotes P < 0.05, n > 31). The difference in recovery times for all strains is depicted as the tolerance index (E) for the heterozygous nej³ or nej^Q7 mutant alleles in comparison to the Canton S or w-/FM7 control flies. Error bars represent SEM (One-way ANOVA w/Dunnett post test: *denotes P < 0.05; **denotes P < 0.01; n = 58 [w-/FM7], 48 [w-, nej³/FM7], 31 [nej^Q7/FM7], 51 [CS]).