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. 2017 Apr 11;10:103. doi: 10.3389/fnmol.2017.00103

Figure 6.

Figure 6

nejire induction produces alcohol resistance. (A,F) Schematic depiction of the 2-day treatment protocol. A heat-shock (HS) inducible transgenic construct in which the Hsp70 promoter drives expression of a nejire cDNA was used to drive expression of CBP, 24 h prior to sedation with either benzyl alcohol (top, A–E) or ethanol (bottom, F–J). (B,C) Recovery curves (B) and average-recovery time (C) from benzyl alcohol sedation of transgenic yw; hs-nej + flies in which CBP was induced (HS) or uninduced (Control). Error bars represent SEM (Student’s t-test: *denotes P < 0.0001, n = 52 [Control], 54 [HS]). (D,E) Recovery curves (D) and average-recovery time (E) from benzyl alcohol sedation of control yw flies lacking the hs-nej + transgene in which a HS or control treatment (Control) was applied. Error bars represent SEM (Student’s t-test: not significant). (G,H) Recovery curves (G) and average-recovery time (H) from ethanol sedation of transgenic yw; hs-nej + flies in which CBP was induced (HS) or uninduced (Control). Error bars represent SEM (Student’s t-test: *denotes P < 0.0001, n = 36 [Control], 36 [HS]). (I,J) Recovery curves (I) and average-recovery time (J) from ethanol sedation of control yw flies lacking the hs-nej + transgene in which a HS or control treatment (Control) was applied. Error bars represent SEM (Student’s t-test: not significant).