TABLE 2.
Susceptibility of fusion to C34 peptide depends on Env expression density in control experiments but not from the LAS
| Env plasmid (amt, μg)a | IC50, nM (control)b | IC50, nM (from LAS) |
|---|---|---|
| JRFL WT (6) | 46.9 | NDc |
| JRFL ΔCT (2) | 306.1 | ND |
| JRFL ΔCT (6) | 727.0 | ND |
| HXB2 WT (10) | 1.9 | 6.0 |
| HXB2 ΔCT (2) | 11.6 | 5.5 |
| HXB2 ΔCT (4) | 25.6 | 5.4 |
a
Amount of Env-expressing plasmid used for transfection of 293T cells.
b
C34 was added either at the beginning of coincubation of E-T cells (control) or after creation of the LAS. The extent of fusion was determined by flow cytometry as described in Materials and Methods.
c
ND, not determined.