FIGURE 2.

(A) C1A growth on alkaline pretreated corn stover. During the initial growth phase (Day 0–2), strain C1A is grown under anaerobic conditions on corn stover, a process that is accompanied by the production of acids and ethanol. Cycloheximide is added after 2 days (red arrow) to initiate the saccharification phase, a process that results in the accumulation of glucose (at a rate of 308 ± 54 μg.h^-1) and xylose (at a rate of 82.6 ± 11.9 μg.h^-1) in the culture media. Green arrow depicts the time of addition of E. coli to initiate the fermentation phase of the process. (B) Addition of E. coli results in the rapid consumption of glucose and xylose in the culture media, and their conversion to ethanol (at a rate of 3907 ± 674.9 μg.h^-1).