Table 1.
Corn stover dry weight (mg) |
Products (mg)2 |
Recovery percentages3 |
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---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
T0 | Tf1 | % Lost | Acids4 | Ethanol | Fungal biomass | Glucose | Xylose | Total sugars | Extracellular proteins5 | g sugar/ g corn stover | g sugar/ g corn stover | Glucose yield6 | Xylose yield7 | Ferment-able sugars yield8 | Sugars: acids ratio9 |
450 | 220 | 51.11 | 89.42 ± 23.7 | 21.29 ± 6.56 | 28.29 ± 4.6 | 55.66 ± 7.42 | 18.93 ± 2.52 | 74.59 | 8.9 | 0.17 | 0.324 | 22.36 | 12.36 | 19.98 | 0.83 |
1Dry weight measured at the conclusion of saccharification phase. 2All products are measured at the conclusion of saccharification phase, with the exception of fungal biomass, which was measured at the end of the growth phase, since hyphal lysis occurred during saccharification. 3Recovery percentages are based on glucan, xylan, and lignin content determined for various lignocellulosic biomass utilized in this experiment and reported in Youssef et al. (2013). 4Values represent cumulative amounts of acetate (79.53 ± 23.53), lactate (5.76 ± 0.07), and formate (4.14 ± 0.06) produced. 5Value corresponds to the free non-adsorbed extracellular proteins measured in the cell-free supernatant. 6Calculated using the formula: [(g glucose produced/g glucan in starting corn stover)] × 100. 7Calculated using the formula: [(g xylose produced/g xylan in starting corn stover)] × 100. 8Calculated using the formula: [g glucose + xylose produced/g cellulose and glucan in starting corn stover] × 100. 9Calculated using the formula: [g glucose + xylose produced/g acetate + formate + lactate produced] × 100.