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. 2017 Apr 11;7:110. doi: 10.3389/fcimb.2017.00110

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Copyright © 2017 Nairz, Schroll, Haschka, Dichtl, Tymoszuk, Demetz, Moser, Haas, Fang, Theurl and Weiss.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Classical innate immune functions are Hfe-independent. The expression of TNF-α (A), IL-1ß (B), IL-6 (C), Nos2 (D), and the p47 phox subunit (E) in the spleen was determined relative to the housekeeping gene Hprt by quantitative RT-PCR. Data were analyzed and presented exactly as described in the legend to Figure 1. All statistically significant differences are indicated. Additional letters represent statistically significant differences (P <0.05) as follows: (c) Hfe^+/+ S. Tm. IA vs. Hfe^+/+ S. Tm. IE. n = 7–10 per group.