FIG. 6.

Tissue distribution analysis by Southern blot. Various tissues were harvested from mice injected with 7.2 × 10¹² vg of AAV8-EF1α-nlslacZ via the tail vein or the portal vein (one mouse each). Total genomic DNA was extracted from tissues, and 10 μg of each DNA was digested with BglI and separated on a 0.8% agarose gel. The vector genomes were detected with a 2.1-kb BglI-BglI lacZ probe. The double-stranded vector genome copy number standards (0 to 30 ds-vg/dge) were prepared as described in the legend to Fig. 4. Abbreviations: b, brain; lu, lung; h, heart; s, spleen; k, kidney; i, intestine; t, testis; p, pancreas; and m, skeletal muscle. In each set of tissues, the left and right lanes represent samples from mice injected via the tail vein and portal vein, respectively. For densitometric analysis, see the Results section.