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Electron microscopic analysis of capsids produced by wt scaffold (top) or the scaffold mutant Δ143-151 (bottom). Capsids were produced in an in vitro assembly reaction and purified by sucrose gradient sedimentation. The peak fractions were analyzed by negative-stain electron microscopy. Note that capsids produced with Δ143-151 are morphologically indistinguishable from capsids produced with the wt scaffold.
