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. 2017 Apr 11;8:521. doi: 10.3389/fpls.2017.00521

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Copyright © 2017 Ahmed, Liu, Xue, Ji, Qi, Guo, Guo and Kang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Transient overexpression of TaDIR1-2 in tobacco (Nicotiana benthamiana). (A) Tobacco leaves were infiltrated with Agrobacterium tumefaciens cells carrying empty vector (EV), Avr1b, (TaDIR1-2+pBin19) or eGFP alone (circles 1-4), or followed 16 h later with a 2nd infiltration of (A). Tumefaciens cells carrying Bax (circles 5-8). Photos were taken 4 days after the 2nd infiltration. (B) The same leaves collected at the same time (4 days after 2nd infiltration) and submerged for 7 days in 100% ethanol when photos were taken. Circles: 1, empty vector (EV); 2, Avr1b; 3, (TaDIR1-2+pBin19); 4, eGFP; 5, empty vector (EV)+Bax; 6, Avr1b+Bax; 7, (TaDIR1-2+pBin19)+Bax; 8, eGFP+Bax. Circles indicate infiltrated areas; same position represents same sample.