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. 2015 Jun 11;5:10394. doi: 10.1038/srep10394

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Cross-linking of VSG in supported membranes. a) As a control for the mSAV experiment, tetravalent neutravidin was added to biotinylated VSG. This leads to rapid crosslinking and immobilization of the protein complexes. The mobile fraction was reduced 7-fold and no diffusion coefficient could be calculated using Soumpasis diffusion algorithm for complexed proteins . The diffusion coefficient and mobile fraction of untreated VSG was normal. Data are means ± SD. b) Treatment of VSG in supported membranes with a VSG-specific antibody causes immediate immobilization of the protein complexes that is slowly reversed after trypsin digestion. Arrows mark the addition of rabbit anti-VSG M1.6 antibody and trypsin, respectively. Diffusion coefficient (□), mobile fraction . The dotted lines represent linear regression and are guidelines for the eye.

Inline graphic — Cross-linking of VSG in supported membranes. a) As a control for the mSAV experiment, tetravalent neutravidin was added to biotinylated VSG. This leads to rapid crosslinking and immobilization of the protein complexes. The mobile fraction was reduced 7-fold and no diffusion coefficient could be calculated using Soumpasis diffusion algorithm for complexed proteins . The diffusion coefficient and mobile fraction of untreated VSG was normal. Data are means ± SD. b) Treatment of VSG in supported membranes with a VSG-specific antibody causes immediate immobilization of the protein complexes that is slowly reversed after trypsin digestion. Arrows mark the addition of rabbit anti-VSG M1.6 antibody and trypsin, respectively. Diffusion coefficient (□), mobile fraction . The dotted lines represent linear regression and are guidelines for the eye.