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. 2016 Nov 22;21(5):881–893. doi: 10.1111/jcmm.13027

Figure 5.

Figure 5

HLC‐5 transplantation improved liver function of CCl4‐injured nude mice (n = 10). (A) The levels of serum parameters ALB, ALT and AST of liver‐injured mice after cell transplantation were measured. (B) H&E staining of liver tissue from mice exposed to different treatments: a) normal; b) CCl4‐injured; c) HLC‐5 transplantation; d) HLC‐7 transplantation; e) saline injection. The scale bar is 50 μm. (C) Sirius red staining of liver tissue from mice exposed to different treatments: a) normal; b) CCl4‐injured; c) HLC‐5 transplantation; d) HLC‐7 transplantation; e) saline injection. The scale bar is 50 μm. (D) The changes in collagen content of different treatment in Sirius red staining. (E) Immunofluorescence staining to locate the transplanted HLC‐5 and HLC‐7: a) white; b) ALB (green); c) the merge of ALB (green); and DAPI (blue). The scale bar is 20 μm. CCl4, carbon tetrachloride.