Table 1.

Primers for RT‐PCR analysis

Gene	Primer (5′ to 3′)	Annealing temperature
GAPDH	F: GCACCGTCAAGGCTGAGAAC	61°C
	R: TGGTGAAGACGCCAGTGGA
ALB	F:ACTGCATTGCCGAAGTGGA	60°C
	R:GCAGCACGACAGAGTAATCAGGA
HNF4A	F:AGCTGCAGATCGATGACAATGAG	61°C
	R:CATACTGGCGGTCGTTGATGTAG
AFP	F:TGCAGCCAAAGTGAAGAGGGAAGA	64°C
	R:CATAGCGAGCAGCCCAAAGAAGAA
TF	F: CCTCCTACCTTGATTGCATCAG	58°C
	R: TTTTGACCCATAGAACTCTGCC
CYP3A4	F: AAGTCGCCTCGAAGATACACA	59°C
	R: AAGGAGAGAACACTGCTCGTG
G6P	F:TAGAGCTGAGGCGGAATGGGAG	60°C
	R: GCTGGAGTCCTGTCAGGCATTGC
CK7	F: GCTGAGGCTGAAGCCTGGTA	61°C
	R: CATCTCTGAAATCTCATTCCGGGTA
PDX1	F: ACTCCACCTTGGGACCTGTTTAGA	62°C
	R: CGAGTAAGAATGGCTTTATGGCAGA
EpCAM	F: GAATGGCAAAGTATGAGAAGGCTGA	61°C
	R: TCCCACGCACACACATTTGTAA
CYP1A1	F: GGAGCTAGACACAGTGATTGGC	60°C
	R: GGTGAAGGGGACGAAGGA
MiR‐122	TGGAGTGTGACAATGGTGTTTG	60–62°C
MiR‐148a	TCAGTGCACTACAGAACTTTGT
MiR‐424	CTTCCCCCCAGTAATCTTCATC
MiR‐542‐5P	TCGGGGATCATCATGTCACGAGA
MiR‐1246	AATGGATTTTTGGAGCAGG
MiR‐30a	TGTAAACATCCTCGACTGGAAG
MiR‐1290	TGGATTTTTGGATCAGGGA