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. 2017 Mar 15;6(4):778–787. doi: 10.1002/cam4.996

Figure 4.

Figure 4

miR‐126 overexpression in B‐cell leukemia cells leads to downregulation of p85β. (A) qRT‐PCR analysis of miR‐126 expression in 697 cell lines infected with a control vector (VO) or a vector containing miR‐126 with or without 48 h induction with doxycycline (DOX) (n = 4). (B) qRT‐PCR analysis of p85β expression in the VO and miR‐126 cell lines with or without 48 h induction with DOX (n = 4). (C) Western blots showing p85β protein expression in the VO and miR‐126 cell lines with or without 48 h induction with DOX. (d) Protein quantification of the Western blot shown in c. p85β expression was normalized to actin and then normalized to the uninduced vector control cells (n = 5). * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001.