Skip to main content
. 2016 Nov 23;21(5):955–967. doi: 10.1111/jcmm.13035

Figure 8.

Figure 8

linc00261 promote the degradation of Slug via enhancing the interaction between GSK3β and Slug. (A) RNA pull‐down assay performed in AGS cells. Biotinylated linc00261 or antisense RNA was incubated with cell extracts, targeted with streptavidin beads and washed, and the associated proteins were resolved on a gel. Western blot analysis detected the specific association of GSK3β and linc00261 (n = 3). (B) Co‐immunoprecipitation detected the interaction of GSK3β and Slug in AGS cells. The 20% of input (cell lysate) and GSK3β or Slug immunoprecipitates were separated by SDS‐PAGE. The specific immunoprecipitation of GSK3β and Slug was confirmed by Western blot analysis (n = 3). (C) Immunoprecipitation assay was performed to detect the interaction between GSK3β and Slug after transfection of linc00261 shRNA in AGS cells. (D) Western blot analysis confirmed that GSK3β‐specific siRNA down‐regulated the protein level of GSK3β. Knockdown of GSK3β abolished the down‐regulation of protein level of Slug induced by linc00261 overexpression.