Figure 5.

Inhibition of Orai1 or SOCE enhances 5‐FU‐induced autophagy and cell death. (A) Cells were treated with Orai1 siRNA (50 nM) for 48 hrs or treated with SKF96365 (20 μM) for 3 hrs prior to 5‐FU (80 μM) incubation. At the end of the experiment, cells were loaded with Fura2/AM probe and subjected to Ca²⁺ imaging experiment. (B) Quantification of fluorescence ratio (340/380). **P < 0.01 versus control, ##P < 0.01 versus 5‐FU, n = 6. (C) Representative Western blotting images showing the expression of LC3B, Beclin‐1, ATG5 and p62. (D) Densitometric analysis of LC3B‐II, Beclin‐1, ATG5 and p62 protein expression was performed. **P < 0.01 versus control, #P < 0.05, ##P < 0.01 versus 5‐FU, n = 5. (E) HepG2 cell viability was analysed by CCK‐8 assay. **P < 0.01 versus control, ##P < 0.01 versus 5‐FU, n = 4.