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. 2016 Nov 23;21(5):904–915. doi: 10.1111/jcmm.13029

Figure 6.

Figure 6

Restoration of Orai1 attenuates 5‐FU‐induced autophagic cell death. (A) Cells were transfected with Orai1 plasmid for 48 hrs prior to 5‐FU (80 μM) incubation for another 48 hrs. Ca2+ imaging experiment was performed as mentioned in methods section. (B) Quantification of fluorescence ratio (340/380). **P < 0.01 versus control, ##P < 0.01 versus 5‐FU, n = 6. (C) Representative Western blotting images showing the expression of LC3B, Beclin‐1, ATG5 and p62. (D) Densitometric analysis was performed using ImageJ analysis software. **P < 0.01 versus control, ##P < 0.01 versus 5‐FU, n = 5. (E) Cell viability was examined by CCK‐8 assay. **P < 0.01 versus control, ##P < 0.01 versus 5‐FU, n = 6.