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. 2017 May;66(5):1001–1011. doi: 10.1016/j.jhep.2017.01.001

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© 2017 European Association for the Study of the Liver. Elsevier B.V. All rights reserved.

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Fig. 2 — Functional polarity defects in Vps33b^fl/fl-AlfpCre mice. (A) Immunostaining for detection of BSEP and (B) MRP2 localisation in Vps33b^fl/fl-AlfpCre and Vps33b^fl/fl liver sections. (C) Plasma and bile levels of cholesterol and (D) phospholipid in Vps33b^fl/fl-AlfpCre and Vps33b^fl/fl mice assessed by fluorometric analysis (n = 5, p = 0.0079 in all tests, Mann Whitney U test). (E) Cumulative biliary levels of CLF and (F) blood levels of CLF measured in Vps33b^fl/fl-AlfpCre and Vps33b^fl/fl (n = 4). All mice were fed a cholic acid supplemented diet and sacrificed at 14–15 weeks of age. Graphs are presented with medians and IQR.