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. 2017 May;66(5):1001–1011. doi: 10.1016/j.jhep.2017.01.001

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© 2017 European Association for the Study of the Liver. Elsevier B.V. All rights reserved.

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Fig. 6 — Bile canaliculus ultrastructure. Transmission electron micrographs of bile canaliculi in (A) Vps33b^fl/fl and (B) Vps33b^fl/fl-AlfpCre mouse liver and in (C) liver of Vps33b^fl/fl-AlfpCre mice injected with 1 × 10¹² vg/mouse of ssAAV2/8-EFS-hVPS33Bco-WPRE vector. (D) Measurements of canalicular membrane length (± SEM) as a factor of canalicular perimeter assessed using ITEM software on mouse hepatocyte electron micrographs (n = 62–90, p <0.0001, One way ANOVA, Bonferroni’s test). All mice were fed a normal chow diet and sacrificed at 14–15 weeks of age.