FIG. 2.

Influences of transfection efficiency and helper virus titer on rAAV yields. (A) 293 cells that had been transfected with pSub201/EGFP at different transfection efficiencies were plated in 35-mm-diameter culture dishes and infected with PS virus (3.72 × 10⁶ TCID₅₀). (B) pSub201/EGFP-transfected cells with a 70% transfection efficiency were infected with different amounts of PS virus, ranging from 0.9 × 10⁶ to 5.4 × 10⁶ TCID₅₀. After 72 h, the cells were harvested, and rAAV vectors were determined by a fluorescent cell assay and expressed in EGFP TU.