FIG. 3.

Determination of rAAV titers by the TCID₅₀ method via coinfection with PS virus. BHK cells (2 × 10⁵/well) were cultured in 24-well plates for 24 h, infected with serial twofold dilutions of rAAV/EGFP for 24 h, and subsequently infected with 10⁶ TCID₅₀ of PS virus. The cells were also infected with either PS virus or rAAV vector alone, which served as negative controls. The cells were observed daily for CPE and were stained with crystal violet 6 days later. Lane 1, PS virus only; lane 2, rAAV vector only; lanes 3 to 6, serial twofold dilutions of rAAV vectors, starting from lane 3, in the presence of PS virus.