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Determination of the optimal amount of PS virus required for rAAV amplification. 293 cells were infected with rAAV/EGFP (103 TU) for 24 h, followed by infection with different amounts of PS virus, ranging from 0.9 × 106 to 5.4 × 106 TCID50. After 72 h, the cells were harvested, and rAAV titers were determined by a fluorescent cell assay and expressed in EGFP TU. The amount of amplification was calculated by dividing the titer of the output virus by that of the input virus.
