Fig. 3.

Itraconazole inhibits the expression of Gli1 in gastric cancer cells. a Expressions of Shh, Ptch, Smo, and Gli1 in MKN45 and AGS cells treated with increasing concentrations of itraconazole for 48 h. Data represent the mean ± SD of 3 determinations and GAPDH is used as the internal control. *P < 0.05; **p < 0.01. b MKN45 and AGS cells are treated with DMSO or indicated concentrations of itraconazole for 48 h, and expressions of major molecules of Hh signaling are determined by immunoblot. A representative blot from three independent experiments is shown. c MKN45 and AGS cells are transiently transfected with Gli1-pGL3, Smo -pGL3 or pGL3-Basic vector and pRL-TK, followed by treatment with 10 μM itraconazole or DMSO. After 48 h, Gli1-pGL3, Smo -pGL3 or pGL3-Basic vector reporter activities were measured and normalized to Renilla luciferase activity. Data represent the mean of three experiments (mean ± SD). *p < 0.05 and **P <0.01 vs DMSO treated cells