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. 2017 Apr 11;17:36. doi: 10.1186/s12896-017-0356-3

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Structure of the plasmids pBUT, pBUT-rO-tMT and pBUT-T7-rO-tMT. Three recombinant plasmids were derived from the expression vector pGETS118 [29, 74], which contained the Pr promoter. To assemble the butanol synthesis pathway, target gene(s) thil (1.2 kb), crt-bcd-etfB-etfA-hbd cluster (4.8 kb), adhe (2.8 kb) and rO-tMT (1.3 kb) or T7-rO-tMT (1.4 kb) were inserted under the Pr promoter, resulting in pBUT (a), pBUT-rO-tMT (b), and pBUT-T7-rO-tMT (c), respectively. All the plasmids include two antibiotic resistances (Tc^r : tetracycline resistance; CAT^r : chloramphenicol resistance)