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. 2017 Apr 11;17:36. doi: 10.1186/s12896-017-0356-3

Table 1.

Strains and plasmids used in this study

Strains and plasmids	Genotype and description^a	Reference or source
E. coli strains
pET-OmTmt	E. coli BL21/PET30a, T7 promoter, f1 origin; ompC-tmt, Km^r	[27]
JM109	endA1, recA1, gyrA96, thi, hsdR17 (rk–, mk+), relA1, supE44, Δ(lac-proAB), [F’ traD36, proAB, laqIqZΔM15].	Promega
PGETS118	E. coli JM109/pGETS118, CAT^r	[75]
BUT1	E. coli JM109/pBUT, CAT^r	This work
BUT2	E. coli JM109/pBUT-rO-tMT, CAT^r	This work
BUT3	E. coli JM109/pBUT-T7-rO-tMT, CAT^r	This work
JM109 (DE3)	JM109 + λ(DE3),Contains an IPTG-inducible gene for T7 RNA polymerase	Promega
PGETS118-DE	E. coli JM109 (DE3)/pGETS118, CAT^r	This work
BUT1-DE	E. coli JM109 (DE3)/pBUT, CAT^r	This work
BUT2-DE	E. coli JM109 (DE3)/pBUT-rO-tMT, CAT^r	This work
BUT3-DE	E. coli JM109 (DE3)/pBUT-T7-rO-tMT, CAT^r	This work
Plasmids
pGETS118	pGETS118SfiI-Pr, Tc^r, CAT^r	This work
pBUT	pGETS118::thil-crt-bcd-etfAB-hbd-adhe, Tc^r, CAT^r	This work
pBUT-rO-tMT	pBUT::rbs-ompC-tmt, Tc^r, CAT^r	This work
pBUT-T7-rO-tMT	pBUT::T7 promoter-ompC-tmt, Tc^r, CAT^r	This work

^aCAT^r, chloramphenicol resistance; Km^r, kanamycin resistant

Tc^r, tetracycline resistance; Pr, Pr promoter; cI, cI cassette